The 2700 SELECT Biochemistry
Analyzer provides vital information about your processes. It provides rapid,
accurate analysis of key nutrients and byproducts, including:
-
Glucose
-
L-Lactate
-
L-Glutamine
-
L-Glutamate
-
Ethanol
-
Lactose
-
Sucrose
-
Galactose
-
Hydrogen peroxide
-
Methanol
-
Choline
Sample
analysis can be done two ways, off-line or on-line.
Off-Line Analysis
For many processes,
off-line analysis is a good method for monitoring nutrients or byproducts,
particularly if consumption or production rates are slow. As a benchtop
Analyzer, the 2700 SELECT is easy to use and provides results quickly—in 60
seconds. Sample volume requirements are low (about 25 µL) which allow you to
run as many samples as you want. And the best feature of all is that no sample
preparation is required (no filtration and no dilution in most cases).
2710 Turntable
Couple the 2700 SELECT with the
2710 Turntable for batch analysis. The 24-position turntable lets you load
samples and walk away. The instrument will automatically analyze them, print
results and send them to a PC.
On-Line Analysis
For processes that require
frequent monitoring and regulation, on-line analysis allows you the freedom to
sample automatically and adjust both nutrient and byproduct
concentrations.
Couple the 2730 Aseptic
Monitoring & Control system with the 2700 SELECT and pull samples directly
from a bioreactor, measure important nutrients and byproducts, and immediately
control the replenishment of nutrients, if needed, while maintaining the
sterility of the process. All system components are sterilizable.
How On-Line Monitoring Works
The 2730 Monitoring and
Control system pulls sample directly from a bioreactor and pumps it to the 2700
SELECT at programmed intervals. After each analysis, an antiseptic reagent,
typically a dilute hypochlorite or hydroxide solution, is flushed through the
sample lines and remains there until the next cycle to assure sterility.
For those bioprocesses in
which loss of cells is a concern, you can connect the system to an on-line
filtration device. You can adjust system parameters, such as flow rate, to
accommodate the needs of the filtration device.
For regulation, connect the
2700 SELECT to a feed pump. When nutrient concentration falls below a
programmed setpoint, the 2700 SELECT will signal the feed pump to add nutrient
to the bioreactor.
Other system variables,
such as sampling interval and concentration setpoint, allow you to customize
the instrument for your process needs. For example, with processes that consume
glucose quickly, you can configure the system to monitor glucose every few
minutes. For other processes that consume nutrients slowly, monitoring every 30
or 60 minutes may be more
appropriate.
Other
Features
RS232 and
analog signal (with 2730) allow data logging with a PC
System can
be set up for closed-loop monitoring and control
Discrete
samples can be run when Analyzer is acting as on-line monitor
On-line
monitor components can be autoclaved
Two
measurement chemistries can be run simultaneously
Measurement
units are selectable: g/L, mg/L, mmol/L, % (w/v)
Chemistries
Unless otherwise stated,
specifications reflect use with YSI-supplied calibration solutions and a 25 µL
sample.
Sample size, calibration
concentration, and endpoints can be changed to accommodate specific
applications.
|
Chemistry
|
Detection
Range (to)
|
Calibration
Point
|
Linearity Check Point
|
Precision
CV
n=10
|
Linearity
0 to cal point
|
Linearity
Cal point to range max
|
Typical Membrane Working Life
|
|
Choline
|
450
mg/L
|
175
mg/L
|
450
mg/L
|
2%
|
±2%
|
±5%
|
7
days
|
|
D-Glucose
Dextrose
(Low Range)
|
9
g/L
at 25 µL sample size
|
2.50
g/L
|
9.0
g/L
at 25 µL
sample size
|
2%
|
±2%
|
±5%
|
21
days
|
|
D-Glucose
Dextrose
(High Range)
|
25
g/L
at 10 µL sample size
|
2.50
g/L
|
25
g/L
at 10 µL
sample size
|
2%
|
±2%
|
±5%
|
21
days
|
|
Ethanol1
|
3.2
g/L
|
2.00
g/L
|
3.20
g/L
|
2%
|
±2%
|
±5%
|
5
days
|
|
Galactose2,3
|
25
g/L
|
N/A
|
N/A
|
N/A
|
N/A
|
N/A
|
10
days
|
|
Hydrogen
Peroxide2
|
600
mg/L
|
N/A
|
N/A
|
N/A
|
N/A
|
N/A
|
21
days
|
|
Lactose
|
25
g/L
|
5.00
g/L
|
25.0
g/L
|
2%
|
±2%
|
±5%
|
10
days
|
|
L-Glutamate
|
10
mmol/L
|
5.00
mmol/L
|
10.0
mmol/L
|
2%
|
±2%
|
±5%
|
7
days
|
|
L-Glutamine4
|
8
mmol/L
|
5.00
mmol/L
|
8.00
mmol/L
|
4%
|
±4%
|
±5%
|
5
days
|
|
L-Lactate
L-Lactic Acid
|
2.67
g/L
|
0.50
g/L
|
2.67
g/L
|
2%
|
±2%
|
±5%
|
14
days
|
|
Methanol5
|
2.50
g/L
|
1.00
g/L
|
2.50
g/L
|
2%
|
±2%
|
±5%
|
5
days
|
|
Sucrose
|
25
g/L
|
5.00
g/L
|
25.0
g/L
|
2%
|
±2%
|
±5%
|
10
days
|
|
1.
Methanol is a potential substrate interference. Specifications are for 10 µL
sample.
2. The user must provide
calibrator solution.
3. Lactose is a potential
substrate interference.
4. Specifications are for the
simultaneous measurement of glutamine and glutamate.
5. Ethanol is a potential
substrate interference. Specifications are for 15 µL sample.
|